Author： Shimada Yuji Hirota Yoshinori Baba Takashi Sugihara Akio Moriyama Shigeru Tominaga Yoshio Terai Tadamasa
Publisher： Springer Publishing Company
ISSN： 0003-021X
Source： Journal of the American Oil Chemists' Society, Vol.76, Iss.6, 1999-06, pp. : 713-716
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Steryl esters of long-chain fatty acids have water-holding properties, and polyunsaturated fatty acids (PUFA) have various physiological functions. Because steryl ester of PUFA can be expected to have both features, we attempted to synthesize steryl esters of PUFA by enzymatic methods. Among lipases used, Pseudomonas lipase was the most effective for the synthesis of cholesteryl docosahexaenoate. When a mixture of cholesterol/docosahexaenoic acid (3:1, mol/mol), 30% water, and 3000 units/g of lipase was stirred at 40°C for 24 h, the esterification extent attained 89.5%. Under the same reaction conditions, cholesterol, cholestanol, and sitosterol were also esterified efficiently with docosahexaenoic, eicosapentaenoic, arachidonic, and -linolenic acids.
Related content
Access to resources
Recommend
By Ferrari R. Esteves W. Mukherjee K.
Journal of the American Oil Chemists' Society, Vol. 74, Iss. 2, 1997-02 ,pp. :
By Ono Masayo Hosokawa Masashi Inoue Yoshikazu Takahashi Koretaro
Journal of the American Oil Chemists' Society, Vol. 74, Iss. 11, 1997-11 ,pp. :