Progesterone supplementation during multiple ovulation treatment in buffalo species ( Bubalus bubalis )

ISSN： 0049-4747

Source： Tropical Animal Health and Production, Vol.42, Iss.6, 2010-08, pp. : 1243-1247

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Abstract

The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n = 7) it was left till day 10, when $$ {\hbox{PG}}{{\hbox{F}}_{{2}\alpha }} $$ was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing. A higher (P < 0.05) number="" of="" corpora="" lutea="" (8.3="" vs.="" 5.7)="" and="" embryo/flushing/buffalo="" (2.3="" vs.="" 1.3)="" were="" recorded="" in="" group="" b="" vs.="" group="" a="" if="" responsive="" buffaloes="" are="" considered="">n = 12) and the number of corpora lutea was highly correlated with the number of embryos (r = 0.65; P < 0.05). in="" conclusion,="" progesterone="" supplementation="" during="" the="" first="" 2 days="" of="" the="" superovulation="" treatment="" seems="" to="" enhance="" the="" recovery="" rate="" in="" buffalo="" species.="" a="" high="" ovulation="" rate,="" associated="" with="" a="" high="" number="" of="" corpora="" lutea,="" can="" represent="" a="" parameter="" for="" estimating="" embryo="" recovery.="">