Author： Ramírez-Ramírez Rocío
Publisher： Springer Publishing Company
ISSN： 0003-6072
Source： Antonie van Leeuwenhoek, Vol.85, Iss.1, 2004-01, pp. : 63-68
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Abstract
A Cr(VI)-resistant yeast was isolated from tanning liquors from a leather factory in Leon, Guanajuato, Mexico. Based on morphological and physiological analyses and the D1/D2 domain sequence of the 26S rDNA, the yeast was identified as Candida maltosa</i>. Resistance of the strain to high Cr(VI) concentrations and its ability to chemically reduce chromium was studied. When compared to the three laboratory yeasts Candida albicans</i>, Saccharomyces cerevisiae</i> and Yarrowia lipolytica</i>, the C. maltosa</i> strain was found to tolerate chromate concentrations as high as 100 </i>g/ml. In addition to this phenotypic trait, the C. maltosa</i> strain showed ability to reduce Cr(VI). Chromate reduction occurred both in intact cells (grown in culture medium or in soil containing chromate) as well as in cell-free extracts. NADH-dependent chromate reductase activity was found associated with soluble protein and, to a lesser extent, with the membrane fraction.
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