Transesterification of alkylphosphate esters by phospolipase D

Author: Aurich I.   Dürrschmidt P.   Hirche F.   Ulbrich-Hofmann R.  

Publisher: Springer Publishing Company

ISSN: 0141-5492

Source: Biotechnology Letters, Vol.19, Iss.9, 1997-01, pp. : 875-879

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Abstract

Phospholipase D from cabbage or Streptomyces chromofuscus catalyses the transesterification of alkylphosphate esters, which are potent antitumor agents. The exchange of choline in octadecylphosphosphocholine by N-(2-hydroxy-ethyl)-N-methylpiperidine with phospholipase D from cabbage is kinetically controlled and amounts to maximum 60% in buffer, 43% in buffer/n-hexane, and 82% in buffer/n-hexane/1-octanol.