Author： Villarreal M.L.
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.23, Iss.16, 2001-08, pp. : 1279-1284
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Abstract
Following a solid phase extraction, GC-MS and GC-FID procedures, the production of three kaurane derivatives (grandiflorenic, kaurenoic and monoginoic acids) was detected in callus and cell suspension batch cultures of Montanoa tomentosa. From different hormonal combinations, the addition of 0.5 mg 2,4-dichlorophenoxyacetic acid l^−1 + 2 mg kinetin l^−1 increased the accumulation of total kaurenoids in 6 months old calluses to 2.1 mg g^−1 dry weight and in cell suspensions cultures up to 0.76 mg g^−1 dry weight. Monoginoic acid, which has not been detected before in leaves of wild plants, accumulated in both in vitro systems.
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