Author： Jutur Pavan P.
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.26, Iss.11, 2004-06, pp. : 929-932
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Abstract
Two novel type II restriction endonucleases, designated as BpaI and BpnI, were isolated from Bacillus pasteurii</i> strain1761 and Bacillus pantothenticus</i> strain1639, respectively. They were partially purified and SDS-PAGE indicated Mr values of 28 and 67 kDa for BpaI, 28 and 48 kDa for BpnI. The partially purified endonucleases hydrolyzed DNA into discrete fragments: pUC18 (2.6 kb for BpaI; 1.8 and 0.8 kb for BpnI), pBR322 (2.5 and 1.8 kb for BpaI; 2.6 and 1.7 kb for BpnI) and Φx174 DNA (3.2 and 2.1 kb for BpaI; 4 and 1.3 kb for BpnI).
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