Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins

Author: Amoroso M.G.  

Publisher: Springer Publishing Company

ISSN: 0141-5492

Source: Biotechnology Letters, Vol.26, Iss.22, 2004-11, pp. : 1731-1737

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Abstract

Developing seeds from Triticum aestivum</i> (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.

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