Author： Park Ki-Bum Oh Suk-Heung
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.28, Iss.18, 2006-09, pp. : 1459-1463
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Abstract
For a foreign glutamate decarboxylase (GAD) to be expressed in Bacillus</i> host system, a recombinant DNA (pLip/LbGAD</i>) was constructed by ligating an LbGAD</i> gene from Lactobacillus brevis</i> OPK-3 into Escherichia coli–Bacillus</i> shuttle vector, pLip. The pLip/LbGAD</i> construct was then transformed into Bacillus subtilis</i>. The culture of the transformed Bacillus</i> strain with the pLip/LbGAD</i> construct had higher GAD activity and -aminobutyric acid (GABA) concentration than those of untransformed Bacillus</i> counterpart. In addition, Chungkukjang, a traditional Korean fermented soybean product prepared by the transformed Bacillus subtilis</i>, contained a significantly higher level of GABA than conventional ones. Thus, by introducing a foreign GAD gene, Bacillus</i> strains have been genetically engineered to produce high levels of GAD and GABA.
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