Functional Characterization of Ketoreductase ( rubN6 ) and Aminotransferase ( rubN4 ) Genes in the Gene Cluster of Streptomyces achromogenes var. rubradiris

Author： Lamichhane Janardan   Liou Kwangkyoung   Lee Hei   Kim Chun-Gyu   Sohng Jae  

Publisher： Springer Publishing Company

ISSN： 0141-5492

Source： Biotechnology Letters, Vol.28, Iss.8, 2006-04, pp. : 545-553

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

ORF’s for rubN6</i> and rubN4</i> have been annotated as thymidine diphosphate glucose 4-ketoreductase and thymidine diphosphate glucose 3-aminotransferase by sequence analysis of the rubradirin biosynthetic gene cluster cloned from Streptomyces achromogenes</i> var. rubradiris</i> NRRL 3061. Both ORFs were heterologously expressed in Escherichia coli</i> as His-tagged fusion proteins. The functionalities of TDP-glucose 4-ketoreductase and TDP-glucose 3-aminotransferase were verified by in vitro</i> enzyme assay, and a biosynthetic pathway for TDP-d</emphasis>-rubranitrose is proposed.

Related content

Functional identification of rub52 gene involved in the biosynthesis of rubradirin

By Maharjan J.  

Biotechnology Letters, Vol. 25, Iss. 11, 2003-06 ,pp. : 909-915 (7)

Springer Publishing Company

Access to resources Recommend Favorite

5-ketoreductase from Streptomyces bingchengensis: overexpression and preliminary characterization

By Wang Xiang-Jing   Wang Cheng-Qin   Sun Xiao-Lin   Xiang Wen-Sheng  

Biotechnology Letters, Vol. 32, Iss. 10, 2010-10 ,pp. : 1497-1502 (6)

Springer Publishing Company

Access to resources Recommend Favorite

Characterization of pbpA and pbp2 Encoding Penicillin-binding Proteins Located on the Downstream of Clavulanic Acid Gene Cluster in Streptomyces clavuligerus

By Ishida Kenji   Hung Trinh   Liou Kwangkyoung   Lee Hei   Shin Chang-Hun   Sohng Jae  

Biotechnology Letters, Vol. 28, Iss. 6, 2006-03 ,pp. : 409-417 (9)

Springer Publishing Company

Access to resources Recommend Favorite

An approach for cloning biosynthetic genes of 2-deoxystreptamine-containing aminocyclitol antibiotics: isolation of a biosynthetic gene cluster of tobramycin from Streptomyces tenebrarius

By Kharel Madan Kumar  

Biotechnology Letters, Vol. 25, Iss. 24, 2003-12 ,pp. : 2041-2047 (7)

Springer Publishing Company

Access to resources Recommend Favorite

Phenotypic characterization of Streptomyces albus genes linked to integrating element by heterologous expression in Streptomyces lividans

By Kwon H-J.  

Biotechnology Letters, Vol. 22, Iss. 5, 2000-03 ,pp. : 367-372 (6)

Springer Publishing Company

Access to resources Recommend Favorite