Author: Zhou S. Shanmugam K. Yomano L. Grabar T. Ingram L.
Publisher: Springer Publishing Company
ISSN: 0141-5492
Source: Biotechnology Letters, Vol.28, Iss.9, 2006-05, pp. : 663-670
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Abstract
A non-recombinant mutant of Escherichia coli</i> B, strain SZ194, was developed that produces over 1 m</i> d</i>-lactate from glucose (or sucrose) in 72 h using mineral salts medium supplemented with 1 mm</i> betaine in simple anaerobic fermentations. Rates and yields were highest at pH 7.5. Yields approached the theoretical maximum with only trace amounts of co-products. Chiral purity of d</i>-lactate was estimated to be 95%. Specific and volumetric productivities for SZ194 in mineral salts medium (pH 7.5) with betaine were equivalent to those in Luria broth.