Fermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain SZ194 using Mineral Salts Medium

Author: Zhou S.   Shanmugam K.   Yomano L.   Grabar T.   Ingram L.  

Publisher: Springer Publishing Company

ISSN: 0141-5492

Source: Biotechnology Letters, Vol.28, Iss.9, 2006-05, pp. : 663-670

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

A non-recombinant mutant of Escherichia coli</i> B, strain SZ194, was developed that produces over 1 m</i> d</i>-lactate from glucose (or sucrose) in 72 h using mineral salts medium supplemented with 1 mm</i> betaine in simple anaerobic fermentations. Rates and yields were highest at pH 7.5. Yields approached the theoretical maximum with only trace amounts of co-products. Chiral purity of d</i>-lactate was estimated to be 95%. Specific and volumetric productivities for SZ194 in mineral salts medium (pH 7.5) with betaine were equivalent to those in Luria broth.