Author： Anil Kumar S. Abyaneh Majid Gosavi S. Kulkarni Sulabha Pasricha Renu Ahmad Absar Khan M.
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.29, Iss.3, 2007-03, pp. : 439-445
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Abstract
Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro</i> has been demonstrated for the first time.</i> The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10–25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.
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