Author： Birichevskaya Larisa Kvach Sergei Sivets Grigorii Kalinichenko Elena Zinchenko Anatoly Mikhailopulo Igor
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.29, Iss.4, 2007-04, pp. : 585-591
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Enzymatic 5′-monophosphorylation and 5′-phosphatidylation of a number of -l- and -d-nucleosides was investigated. The first reaction, catalyzed by nucleoside phosphotransferase (NPT) from Erwinia herbicola</i>, consisted of the transfer of the phosphate residue from p</i>-nitrophenylphosphate (p</i>-NPP) to the 5′-hydroxyl group of nucleoside; the second was the phospholipase d (PLD)-catalyzed transphosphatidylation of l-α-lecithin with a series of -l- and -d-nucleosides as the phosphatidyl acceptor resulted in the formation of the respective phospholipid-nucleoside conjugates. Some -l-nucleosides displayed similar or even higher substrate activity compared to the -d-enantiomers.
Related content
Access to resources
Recommend
Enzymatic esterification of bixin by l-ascorbic acid
By Humeau C. Rovel B. Girardin M.
Biotechnology Letters, Vol. 22, Iss. 2, 2000-01 ,pp. :
Enzymatic production of 18-β-glycyrrhetinic acid from Glycyrrhiza glabra L.
By Morana A.
Biotechnology Letters, Vol. 24, Iss. 22, 2002-11 ,pp. :