Author： Chen Ji-Ming Yaiw Koon Yu Meng Wang Lin-Fa Wang Qing-Hua Crameri Gary Wang Zhi-Liang
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.29, Iss.6, 2007-06, pp. : 871-875
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Abstract
The genus Henipavirus</i> in the family Paramyxoviridae</i> compromises two newly identified dangerous pathogens, Nipah virus</i> and Hendra virus</i>. Phosphoprotein of the two viruses is one of the major immunodominant antigens and the most divergent protein in the viral genomes. We have now expressed two pairs of truncated phosphoproteins of the two viruses in Escherichia coli</i> in a soluble form using a vector tailored from pET32a. The truncated recombinant phosphoproteins were purified with His-Tag affinity chromatography and their antigenicity was determined by western blotting and ELISA. The longer pair of truncated recombinant phosphoproteins, covering amino acid residues 4–550, was more antigenic than the shorter one and of potential utility in the serological differentiation of henipavirus infections.
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