Author： Rechmann Henrik Friedrich Andrea Forouzan Dara Barth Stefan Schnabl Heide Biselli Manfred Boehm Robert
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.29, Iss.6, 2007-06, pp. : 971-977
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Abstract
The feasibility of oxygen transfer rate (OTR) measurement to non-destructively monitor plant propagation and vitality of photosynthetically active plant in vitro culture of duckweed (Wolffia australiana</i>, Lemnaceae) was tested using Respiration Activity Monitoring System (RAMOS). As a result, OTR proofed to be a sensitive indicator for plant vitality. The culture characterization under day/night light conditions, however, revealed a complex interaction between oxygen production and consumption, rendering OTR measurement an unsuitable tool to track plant propagation. However, RAMOS was found to be a useful tool in preliminary studies for process development of photosynthetically active plant in vitro cultures.
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