Author： Pan Hai-Xue
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.33, Iss.4, 2011-04, pp. : 733-739
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Abstract
Spinosyns A and D are novel macrolide secondary metabolites containing two deoxysugars. Each of the sugars is derived from a common intermediate, which is biosynthesized sequentially by NDP-glucose synthase and glucose dehydratase. Systematical duplication of gtt</i> and gdh</i> was carried out by genetic engineering in Saccharopolyspora spinosa</i>. Introduction of additional gtt</i> and gdh</i> genes under the control of PermE</i>* promoter showed significant increase on their transcript levels. The production of spinosad was increased by over threefold to reach 800 g/ml in the recombinant strain SIPI-M2093.
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