Author： Panagiotou Gianni Granouillet Pierre Olsson Lisbeth
Publisher： Springer Publishing Company
ISSN： 0175-7598
Source： Applied Microbiology and Biotechnology, Vol.72, Iss.6, 2006-10, pp. : 1117-1124
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
The production of a battery of arabinoxylan-degrading enzymes by the fungus Penicillium brasilianum</i> grown on brewer’s spent grain (BSG) under solid-state fermentation was investigated. Initial moisture content, initial pH, temperature, and nitrogen source content were optimized to achieve maximum production of feruloyl esterase, xylanase, and α-l-arabinofuranosidase. Under the optimum growth conditions (80% moisture, pH 6, 26.5°C, and 5 g/l nitrogen source), the maximum level of feruloyl esterase (1,542 mU/g BSG) was found after 196 h, whereas xylanase (709 U/g BSG) and ArabF activity (3,567 mU/g BSG) were maximal after 108 h and 96 h, respectively. Based on substrate utilization data, the feruloyl esterases produced by P. brasilianum</i> was anticipated to subclass B. A crude enzyme (CE) preparation from P. brasilianum</i> culture grown on BSG was tested for the release of hydroxycinnamic acids and pentoses from BSG. The P. brasilianum</i> CE produced in this work contains a balance of cell wall-modifying enzymes capable of degrading arabinoxylan of BSG by more than 40%.
Related content
By Topakas Evangelos Katapodis Petros Kekos Dimitris Macris Basil Christakopoulos Paul
World Journal of Microbiology and Biotechnology, Vol. 19, Iss. 2, 2003-03 ,pp. :
Access to resources
Recommend
By Sun Haiyan Ge Xiangyang Zhang Weiguo
World Journal of Microbiology and Biotechnology, Vol. 23, Iss. 5, 2007-05 ,pp. :