Colorimetric method for a rapid detection of oxygenated aromatic biotransformation products

Author: Quintana M.G.   Didion C.   Dalton H.  

Publisher: Springer Publishing Company

ISSN: 0951-208X

Source: Biotechnology Techniques, Vol.11, Iss.8, 1997-01, pp. : 585-587

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Abstract

The quantitative production of the oxygenated products from the biotransformation of aromatic substrates can be detected using a very simple and rapid colorimetric test. The method is based on Gibbs' reagent (2,6-dichloroquinone-4-chloroimide) and has been developed for routine spectrophotometric or microtitre plate assay allowing the detection of products with a sensitivity as low as 5 mM.