Author： Tsai S.W. Jeng M.J. Tsay R.Y. Wang Y.J.
Publisher： Springer Publishing Company
ISSN： 0951-208X
Source： Biotechnology Techniques, Vol.12, Iss.1, 1998-01, pp. : 21-23
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Spherical gel beads of collagen/alginate were prepared by discharging droplets of a mixture containing collagen (1.07-1.9 mg/ml) and alginate (1.2-1.5% w/v) into 1.5% w/v CaCl2 solution at 4°C. Collagen in the gel beads was reconstituted by raising the temperature to 37°C after alginate was liquefied by citrate. Scanning electron microscopy of the beads revealed the characteristic fibrous structure of collagen. To demonstrate the application of this new technique in cell culture, GH3 rat pituitary tumor cells were entrapped and grown in the gel beads. The immobilized cells proliferated to a density of 1.95 x 106 cell/ml which is about an order of magnitude higher than that grown in the alginate beads.
Related content
Access to resources
Recommend
Alginate beads as an affinity material for alpha amylases
Bioseparation, Vol. 7, Iss. 3, 1998-01 ,pp. :