Author: Å kesson M.
Publisher: Springer Publishing Company
ISSN: 0951-208X
Source: Biotechnology Techniques, Vol.13, Iss.8, 1999-08, pp. : 523-528
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Abstract
A strain-independent feeding strategy for fed-batch cultures of Escherichia coli is presented. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.
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