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Stem Cell Genes Synergism: Elucidation of the Mechanistic Basis of Response Induced by Cytokine Combination (IL-3, FLT-3, and SCF)

Abstract

1. Introduction

2. Methods

2.1. Microarray Data Source

2.2. Protein-Protein Interaction (PPI) Data

2.3. Gene Expression Profile Analysis

2.4. Analysis of Pathway Crosstalk

3. Results

3.1. Overview of Gene Expression Analysis

3.2. Identification of Molecular Signatures

Table 1. Gene ontology of differentially expressed up-regulated genes

(Table 1 continued on next page.)

(Table 1 continued on next page.)

Table 2. Gene ontology of differentially expressed down-regulated genes

3.3. Signatures of Proliferation and Self-Renewal

3.4. Protein-Protein Interaction Networks for Predicting Functional Domains

4. Discussion

Table 3. Functional biomarkers of targeted genes and their interacting partners

Table 4. The gene / proteins which were found to be i) co-expressed and predicted (ii) physical interaction and predicted

Conclusion

Acknowledgment

Conflict of Interest

Supplementary Material

References

Effect of Culture Conditions on Colony-Forming Ability of Stem Cells from Human Exfoliated Deciduous Teeth

Abstract

Introduction

Materials and Methods

Tooth Sample Collection

Isolation and Culturing of SHED

Immunophenotypic Characterization of SHED

Differentiation

Colony-Forming Unit-Fibroblast (CFU-F) Assay

Culturing Time

Seeding Density

FBS Supplement

Ascorbic acid and L-ascorbic acid 2-phosphate supplement

Analysis

Statistical Analysis

Results

Isolation and Characterization of SHED

Optimal Culture Time for Colony Forming Unit-Fibroblast (CFU-F) Assay

Table 1. Number of SHED colonies in day 12-14

Table 2. Seeding density and colony number of SHED

Effect of Cell Seeding Density on the Formation of SHED Colonies

Selection of optimal supplemented substrates for CFU-F assay

Fetal Bovine Serum

Ascorbic acid and L-ascorbic acid 2-phosphate

Discussion

Acknowledgments

Ethical Compliance

References

In Vitro Proliferation of Human Haematopoietic Stem Cells by Andrographis paniculata (Burm.f.) Nees Extracts

Abstract

1. Introduction

2. Materials and Methods

2.1. Preparation of Medium

2.2. Thawing of Cryopreserved Cells

2.3. Morphological Identification of Cultured Cells through Leishman’s Staining

2.4. Identification ofCD34 Surface Marker on HSCs

2.5. Flowcytometry Analysis of HSCs

2.6. Scanning Electron Microscopy

2.7. Preparation of Nelavembu Extracts

2.8. In Vitro Proliferation Assay of HSCs

2.9. Preparation of TPO Receptor Structure

2.10. Preparation of Ligand

2.11. Docking

Table 1. Represents 20 model of receptor structures for Thrombopoietin build with modeler9v9

3. Results

3.1. Characterization of CD34+ Cells

3.2. In Vitro Assay

3.3. In Silico Analysis

Table 2. Autodock results of receptor model 9 with andrographolide, eltrombopag and Pegacaristim

Table 2A. Binding energies of Andrographolide ranked based on their energy values

Table 2B. Binding energies of Eltrombopag ranked based on their energy values

Table 2C. Binding energies of Pegacaristim ranked based on their energy values

Table 3. Molecular docking of Model 9 with its ligands

3A) Andrographolide

3B) Eltrombopag

3C) Pegacaristim

4. Discussion

Conclusion

Acknowledgment

References

Intrinsic vs. Extrinsic Regulatory Mechanisms of Lung Stem Cell Biology and Behavior

References

Quality Management in the Manufacture of Stem-Cell-Based-Products

Abstract

Background

Basic Concepts of Quality Management System

The QMS Components

1. Vision and Mission of the Organization

2. Quality Manual and Quality Policy of the Organization

3. Customer Focus

4. Leadership

5. People Engagement

6. Various Approaches for Systematic Quality

7. Continuous Improvement

8. Evidence-Based Decision Making

9. Relationship Management

10. Quality Control (QC)

11. Need for Reference Centers (Centers of Excellence), Benchmarks and International Standards

12. Cost Considerations

13. Validation Plans

14. Challenges with Scalability, and Animal -Free Reagents

QMS Implementation

Conclusion

Conflict of Interest

Acknowledgments

References