Determination of ochratoxin A in pig liver-derived pa^te´s by high-performance liquid chromatography

Author: Jiménez A.M.   de Cerain A.   Gonzalez-Peñas E.   Bello J.  

Publisher: Taylor & Francis Ltd

ISSN: 1464-5122

Source: Food Additives and Contaminants, Vol.18, Iss.6, 2001-06, pp. : 559-563

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Abstract

A solid phase extraction procedure followed by HPLC analysis with fluorescence detection has been developed for ochratoxin A (OTA) in pâtés derived from pig liver. After a previous extraction of OTA with 60% acetonitrile, all the samples were purified through C8 columns. The percentage recovery was 85.7% and the lower limits calculated for accurate detection and quantitation were 0.56ng/g and 0.84ng/g respectively. The HPLC procedure showed a good linearity in the interval of equivalent OTA concentrations of 0.84 to 3ng/g. Values <10% were="" obtained="" for="" precision="" in="" hplc="" determinations="" performed="" (n="3)" and="" (n="9)." stability="" of="" calibration="" standards="" and="" samples="" during="" the="" analytical="" procedure="" was="" also="" demonstrated.="" this="" method="" was="" successfully="" applied="" to="" 38="" pig-derived="" pâtés="" and="" three="" samples="" were="" found="" to="" be="" positive="" with="" ota="" levels="" above="" the="" detection="" limit.="" the="" highest="" concentration="" (1.77ng/g)="" has="" been="" found="" in="" a="" home-made="" pâté.="">