

Author: Mai Hao T. Brodie Darlene L. Meyers Martin B. Baldo Amelia L. Krantz Zoe Weisz Adrian
Publisher: Taylor & Francis Ltd
ISSN: 1464-5122
Source: Food Additives and Contaminants, Vol.23, Iss.6, 2006-06, pp. : 547-551
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Abstract
An HPLC method was developed for the quantitative determination of 2,4,6-triiodoresorcinol (I3R), 2-(2′,4′-dihydroxy-3′,5′-diiodobenzoyl)benzoic acid, resorcinol, phthalic acid, and sodium iodide in the colour additive FD&C Red No. 3 (erythrosine) (R3). Due to the fast decomposition of I3R in aqueous solutions, the dye portions analysed were dissolved in methanol and the determinations were performed in the freshly-made solutions. The HPLC method is rapid (50min total analysis cycle, ∼16min to detect I3R and the other intermediates), simple to implement, and generates only small amounts of solvent waste. It was found to be applicable for use in routine batch-certification as shown by the analysis of test portions from 24 lots of R3 submitted for US-certification by domestic and foreign manufacturers during the past three years.
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