Author： Sulbarán-de-Ferrer Betzabé Aristiguieta Marielena Dale Bruce Ferrer Alexis Ojeda-de-Rodriguez Graciela
Publisher： Humana Press, Inc
ISSN： 0273-2289
Source： Applied Biochemistry and Biotechnology, Vol.105, Iss.1-3, 2003-03, pp. : 155-164
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Abstract
Rice straw pretreated with liquid anhydrous ammonia was hydrolyzed with cellulase, cellobiase, and hemicellulase. Ammonia-processing conditions were 1.5 g of NH3/g of dry matter, 85°C, and several sample moisture contents. There were four ammonia addition time (min)-processing time (min) combinations. Sugars produced were analyzed as reducing sugars (dinitrosalicylic acid method) and by high-performance liquid chromatography. Monomeric sugars increased from 11% in the nontreated rice straw to 61% of theoretical in treated rice straw (79.2% conversion as reducing sugars). Production of monosaccharides was greater at higher moisture content and was processing time dependent. Glucose was the monosaccharide produced in greater amounts, 56.0%, followed by xylose, arabinose, and fructose, with 35.8, 6.6, and 1.4%, respectively.
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