The Effect of Bioindicator Preparation and Storage on Thermal Resistance of Bacillus stearothermophilus Spores

Author： Vessoni Penna T.C.   Ishii M.   Machoshvili I.A.   Marques M.  

Publisher： Humana Press, Inc

ISSN： 0273-2289

Source： Applied Biochemistry and Biotechnology, Vol.99, Iss.1-3, 2002-03, pp. : 525-538

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Abstract

Paper strips inoculated with spores of Bacillus stearothermophilus ATCC 7953 were conventionally dried (lot 1) and lyophilized (lot 2); stored in defined environments of 32 and 86% relative humidity at 10, 25 and 33°C for 210 d; and submitted to moist heat treatments at 121°C. A significant decrease in thermal resistance from initial starting levels was found for lyophilized bioindicators stored at 86% relative humidity. The respective average D121°C values were 1.55 ± 0.05 and 1.37 ± 0.10 min for lyophilized bioindicators stored at 32 and 86% relative humidity; and 1.65 ± 0.15min and 1.57 ± 0.11 min for dried bioindicators stored in the same environments.

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