The Effect of Composition of Parenteral Solution on the Thermal Resistance of Bacillus stearothermophilus and Bacillus subtilis Spores

Author： Vessoni Penna T.C. Ishii M. Machoshvili I.A. Marques M.

Publisher： Humana Press, Inc

ISSN： 0273-2289

Source： Applied Biochemistry and Biotechnology, Vol.99, Iss.1-3, 2002-03, pp. : 539-552

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121°C) and Bacillus subtilis ATCC 9372 (Tr = 104.5°C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D_121°C value of 1.31 to 3.14 min, in glucophysiologic and Ringer's solutions respectively. For B. subtilis, D_104.5°C value increased from 0.69 to 1.37 min, in Ringer's (pH=5.91) and 50% glucose (pH 3.05) solutions respectively. The z value ranged from 7.95°C (20% mannitol solution) to 13.14°C (50% glucose solution), corresponding to an activation energy (Ea) of 81.48 and 49.30 kcal/ mol, respectively.