Author： Ciftci Mehmet
Publisher： Informa Healthcare
ISSN： 1475-6366
Source： Journal of Enzyme Inhibition and Medicinal Chemistry, Vol.20, Iss.5, 2005-10, pp. : 485-489
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Abstract
Inhibitory effects of some drugs on glucose 6-phosphate dehydrogenase from the erythrocytes of rainbow trout ( Oncorhynchus mykiss Walbaum, 1792) were investigated. The enzyme was purified 2488-fold in a yield of 76.8% using ammonium sulfate precipitation and 2′,5′-ADP Sepharose 4B affinity gel at 4°C. The drugs pental sodium, MgSO 4 , vancomycin, metamizol, marcaine, and prilocaine all exhibited inhibitory effects on the enzyme. While MgSO 4 (K i = 12.119 mM), vancomycin (K i = 1.466 mM) and metamizol (K i = 0.392 mM) showed competitive inhibition, pental sodium (K i = 0.748 mM) and marcaine (K i = 0.0446 mM) displayed noncompetitive inhibition.
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