Effect of different Solvent Polarity on Extraction of Phenolic Compounds from Algerian Rhanterium adpressum Flowers and their Antimicrobial and Antioxidant Activities

Publisher： Bentham Science Publishers

E-ISSN： 1872-3136 |8|1|43-50

ISSN： 2212-7968

Source： Current Chemical Biology, Vol.8, Iss.1, 2014-11, pp. : 43-50

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Abstract

The aim of this study is to evaluate by in vitro tests the antioxidant and antimicrobial effects of various extractsfrom Algerian Rhanterium adpressum flowers. The effect of different solvents; (methanol-water) and (acetone-water) withvarying polarities concentration (50-90%, v/v) on the extraction of phenolic compounds was studied. Total phenolic content(TPC), total flavonoid content (TFC) and proanthocyanidins content assays were used for the determination of phenoliccompounds. The results of antioxidant tests assessed by DPPH, ABTS and phosphomolybdenum showed that flowersextracts of Rhanterium adpressum expressed a considerable activity. Results of antimicrobial activity using agar hole diffusionmethod indicated moderate antibacterial effect. Screening analysis of phenolic content in these extracts suggestedthat these biologically active components are responsible for the antioxidant and antimicrobial activities.