α-Glucosidase Inhibitory Activity of Polyphenols from the Burs of Castanea mollissima Blume

Author： Zhang Jianwei Zhao Shan Yin Peipei Yan Linlin Han Jin Shi Lingling Zhou Xiaojing Liu Yujun Ma Chao

Publisher： MDPI

E-ISSN： 1420-3049|19|6|8373-8386

ISSN： 1420-3049

Source： Molecules, Vol.19, Iss.6, 2014-06, pp. : 8373-8386

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Abstract

Polyphenol extracts from the burs of Castanea mollissima Blume (CMPE) exhibited potential antioxidant and hypoglycemic activities. The α-glucosidase inhibitory activities of CMPE were assessed as a means of elucidating the mechanism behind its hypoglycemic activities. In vitro studies showed that CMPE significantly inhibited both yeast α-glucosidase, through a noncompetitive mode with an IC₅₀ of 0.33 μg/mL, and rat intestinal α-glucosidase. In vivo studies revealed that oral administration of CMPE at doses of 600 mg/kg significantly reduced postprandial blood glucose levels by 27.2% in normal rats following sucrose challenges. Gel permeation chromatography revealed that CMPE exhibited typical characteristics of high-molecular-mass polymers with mean (Mn) and weight (Mw) average molecular weights of 35.4 and 50.7 kDa, respectively, and a polydispersity (Mw/Mn) of 1.432. Acid hydrolysis analysis indicated the presence of ellagitannins. These data suggest that CMPE, enriched with ellagitannins, would be an efficacious dietary supplement for diabetes management through the inhibition of alpha-glucosidase.