Author: Yamaguchi Akira
Publisher: Springer Publishing Company
ISSN: 1618-2642
Source: Analytical and Bioanalytical Chemistry, Vol.386, Iss.3, 2006-10, pp. : 627-632
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Abstract
The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). based="" on="" the="" difference="" in="" the="" time-course="" responses="" of="" shg="" and="" fluorescence="" spectroscopies="" for="" all="" of="" the="" hemicyanine="" dyes="" tested,="" we="" propose="" that="" hydration="" of="" the="" sulfonate="" group="" is="" mainly="" responsible="" for="" the="" low="" diffusivity="" of="" the="" zwitterionic="" hemicyanine="" dye.=""></2000 s).>
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