The effect of the anaesthetic, Propofol, on in-vitro oocyte maturation, fertilization and cleavage in mice.

ISSN： 1460-2350

Source： Human Reproduction, Vol.12, Iss.6, 1997-06, pp. : 1271-1274

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Abstract

Propofol is a common anaesthetic agent used for oocyte retrieval procedures during in-vitro fertilization (IVF). The effect of Propofol in vitro on mouse oocyte maturation, fertilization and embryo cleavage was studied. In this study, 551 cumulus-free and 222 cumulus-enclosed oocytes from mice stimulated with pregnant mare's serum gonadotrophin (PMSG) were incubated for 30 min in medium containing 0, 100, 1000 or 10,000 ng/ml of Propofol prior to in-vitro maturation. Also, 325 cumulus-enclosed oocytes from mice stimulated to ovulate with PMSG/human chorionic gonadotrophin (HCG) were incubated for 30 min in similar concentrations of Propofol prior to IVF. Maturation, fertilization and cleavage rates were compared. A significant decrease in the in-vitro maturation rate was observed only when the cumulus-free and cumulus-enclosed oocytes were exposed to 10,000 ng/ml Propofol (P < 0.0074="" and="" p="">< 0.0001="" respectively).="" fertilization="" and="" embryo="" cleavage="" rates="" were="" not="" significantly="" different="" compared="" with="" the="" controls.="" these="" findings="" give="" some="" reassurance="" with="" respect="" to="" human="" ivf.="" however,="" further="" studies="" on="" the="" potential="" effects="" of="" propofol="" on="" implantation="" and="" pregnancy="" outcome="" following="" ivf="" are="" needed.="">