Arthropod Predation: A Simplified Immunodot Format for Predator Gut Analysis

Author: GREENSTONE MATTHEW H.   TROWELL STEPHEN C.  

Publisher: Entomological Society of America

ISSN: 1938-2901

Source: Annals of the Entomological Society of America, Vol.87, Iss.2, 1994-03, pp. : 214-217

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Abstract

We describe a new immunodot format which retains the speed, sensitivity, economy, and specificity of the previously published assay but eliminates some apparatus and is simpler to perform. Assays are run on nitrocellulose membranes onto which predator homogenates are applied directly; they use monoclonal antibodies to prey egg or larval proteins, and can be interpreted by eye. The assay detects the remains of a single egg or fifth instar of Helicoverpa zea (Boddie) in the gut of a second-instar or adult Podisus maculiventris (Say), respectively.