Author： Haas Jens Mueller-Kuller Thea Klein Stefan Engels Joachim
Publisher： Taylor & Francis Ltd
ISSN： 1525-7770
Source： Nucleosides, Nucleotides & Nucleic Acids, Vol.26, Iss.6-7, 2007-06, pp. : 865-868
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
We recently reported that a 1'-deoxy-1'-(4,6-difluoro-1H-benzimidazol-1-yl)-2'-(β-aminoethyl)-β-d-ribofuranose nucleoside appears to be a universal nucleoside which does not differentiate between the four natural nucleosides A, C, G, and U in duplexes. Moreover, ribozymes modified with this nucleoside analog showed a better or at least equal catalytic activity relative to Watson-Crick mismatches.[1] Due to these data, we investigated the ability of this compound to tolerate Watson-Crick mismatches in order to avoid HIV escape mutations in RNA interference. The influence of this nucleoside analog on siRNA efficiency was analyzed with a proven siRNA targeting GFP.
Related content
By Brazier John A. Shibata Takayuki Townsley John Taylor Brian F. Frary Elaine Williams Nicholas H. Williams David M.
Nucleic Acids Research, Vol. 33, Iss. 4, 2005-01 ,pp. :
Access to resources
Recommend
By Hogrefe Richard Lebedev Alexandre Zon Gerald Pirollo Kathleen Rait Antonina Zhou Qi Yu Wei Chang Esther
Nucleosides, Nucleotides & Nucleic Acids, Vol. 25, Iss. 8, 2006-09 ,pp. :