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Folding of 16S rRNA in a Signal‐Producing Structure for the Detection of Bacteria

Publisher： John Wiley & Sons Inc

ISSN： 0044-8249

Source： ANGEWANDTE CHEMIE, Vol.125, Iss.40, 2013-09, pp. : 10780-10782

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

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Abstract

64 DNA‐Stränge hybridisieren an 16S‐rRNA unter Bildung von 32 katalytischen Desoxyribozym‐Kernen und erzeugen so ein Fluoreszenzsignal. Dieser Ansatz ermöglicht den Nachweis von 0.6 pM 16S‐rRNA, oder etwa 3×104 Bakterienzellen, ohne Polymerasekettenreaktion.

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