EFFECT OF UVA IRRADIATION ON B16 MELANOMA CELLS IN MONOLAYER CULTURE

E-ISSN： 1346-8138|9|5|409-412

ISSN： 0385-2407

Source： THE JOURNAL OF DERMATOLOGY, Vol.9, Iss.5, 1982-10, pp. : 409-412

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Abstract

ABSTRACTB16 murine melanoma cells in monolayer culture were exposed to a single dose of long wave ultraviolet light (UVA). The number of viable melanoma cells was decreased at 10 hrs post irradiation in parallel to the increase in UVA dose (104~107 ergs/cm2). After ten hours, the cultured cells entered the logarithmic growth phase. The doubling time of the cells exposed to the low dose of UVA was similar to that of nonirradiated cells, but the exposure to higher doses of UVA (105~106 ergs/cm2) elongated the doubling time of these cells. Still higher doses of UVA exposure (107 ergs/cm2) caused no further elongation of doubling time.The tyrosinase activity of these melanoma cells was also measured using an oxygen electrode. The tyrosinase activity of each irradiated cell at ten hours was significantly increased, but no significant increase of tyrosinase activity per mg protein was observed, since protein content per cell increased at the same time. The increase in tyrosinase activity was still observed after ten hours in cells exposed to higher amounts of UVA. These results suggest that UVA repressed cell division and induced protein synthesis to prepare for melanogenesis.