Stretching tethered DNA chains in shear flow

Author: Ladoux B.   Doyle P. S.  

Publisher: Edp Sciences

E-ISSN: 1286-4854|52|5|511-517

ISSN: 0295-5075

Source: EPL (EUROPHYSICS LETTERS), Vol.52, Iss.5, 2010-03, pp. : 511-517

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Abstract

We discuss the stretching of tethered chains subject to a shear flow. Fluorescence microscopy measurements of the extension of sheared tethered DNA, “model polymers”, of various lengths are presented. The tethered chains approach full extension as the flow rate is increased, but very slowly. We show that a general theory to understand the large deformation of any sheared tethered chain must include both the correct nonlinear force-extension relation and chain fluctuations transverse to the flow direction, which themselves depend on the nonlinear elasticity of the chain. Direct comparison of derived scaling laws to simulations and experiments support our theory.