Author: Sahay S.
Publisher: Springer Publishing Company
ISSN: 0959-3993
Source: World Journal of Microbiology and Biotechnology, Vol.15, Iss.6, 1999-12, pp. : 733-735
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Psyllium (Isubgol), the mucilage husk of Plantago ovata, was successfully used as an alternative gelling agent (5% w/v as ground husk for pouring medium and 4% w/v as ground husk in combination with 0.5% w/v agar for slant) for microbial culture. Most of the undesirable features of psyllium-gelled media (slant as well as pouring) were removed by adding the u.v.-treated (20 min) or oven-sterilized (120 ^°C for 1 h) psyllium as ground husk to autoclaved medium at 50–60 ^°C under aseptic condition just before pouring.