Heterofermentative metabolism of glucose and ribose and utilisation of citrate by the smooth biotype of Lactobacillus amylovorus NCFB 2745

Author: Whitley K.  

Publisher: Springer Publishing Company

ISSN: 0003-6072

Source: Antonie van Leeuwenhoek, Vol.75, Iss.3, 1999-04, pp. : 217-223

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Lactobacillus amylovorus NCFB 2745 exhibits a rough colony morphology, ferments glucose homofermentatively and cannot utilise ribose. After five transfers in de Man Rogosa and Sharpe media (containing glucose and citrate) Lb. amylovorus 2745 appears smooth on agar plates; smooth cultures reverted to rough by culturing in aerobic conditions. The smooth type shows patterns of fermentation that are typical of a heterofermentative lactobacillus. Thus, the smooth morphotype produces CO_2 and ethanol in addition to lactate and is able to ferment ribose. The switch in metabolism to the smooth form is accompanied by an increase in phosphoketolase and a reduction in aldolase enzyme activities. Citrate also has effects on growth rates and end-metabolites.