Author： Ueji S-i.
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.25, Iss.1, 2003-01, pp. : 83-87
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Abstract
Semi-purified lipases from Candida rugosa</i>, Pseudomonas cepacia</i> and Alcaligenes</i> sp. were chemically modified with a wide range of hydrophobic groups such as benzyloxycarbonyl, p</i>-nitrobenzyloxycarbonyl, p</i>-methoxybenzyloxycarbonyl, t</i>-butoxycarbonyl, lauroyl and acetyl moieties. The Candida rugosa</i> lipase MY modified with the benzyloxycarbonyl group (modification ratio = 84%) brought about a 15-fold increase in enantioselectivity (E</i> value) towards the hydrolysis of racemic butyl 2-(4-ethylphenoxy)propionate in an aqueous buffer solution, although the enzymatic activity was decreased. The origin of the enantioselectivity enhancement by chemical modification of the lipase is attributed to a significant deceleration in the initial reaction rate for the incorrectly binding enantiomer.
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