Author： Yamazaki T. Tsugawa W. Sode K.
Publisher： Springer Publishing Company
ISSN： 0141-5492
Source： Biotechnology Letters, Vol.21, Iss.3, 1999-03, pp. : 199-202
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Abstract
A hetero-oligomeric glucose dehydrogenase (GDH) from a moderate thermophilic bacterium, SM4 was cross-linked with glutaraldehyde (GA) and it now showed only one optimum temperature for reaction at around 65 °C, which approximately follows the Arrhenius equation. The native enzyme had shown optima at both 45 °C and 75 °C. In addition to the alteration of the optimum temperature for reaction, GA cross-linked GDH retained more than 90% of its initial activity even after 30 min of incubation at 65 °C.
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