Author： Adem Sevki Ciftci Mehmet
Publisher： Informa Healthcare
ISSN： 1475-6366
Source： Journal of Enzyme Inhibition and Medicinal Chemistry, Vol.22, Iss.6, 2007-12, pp. : 751-754
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Inhibitory effects of some drugs were investigated on human erythrocyte 6-phosphogluconate dehydrogenase obtained with a 6552-fold purification in a yield of 78% using 2', 5'-ADP Separose 4B affinity gel. Which on SDS polyacrylamide gel electrophoresis showed a single band. Larnoxicam, metronidazole, imipenem, ornidazole, vancomycin, clindamycin, and amoxicillin exhibited inhibitory effects on the enzyme in vitro with IC50 values of 0.17, 0.23, 0.43, 21.79, 46.39, 117.43 and 287.35 mM, and the Ki constants 0.40 ± 0.04, 0.57 ± 0.06, 0.77 ± 0.11, 42.40 ± 2.89, 65.60 ± 4.03, 130.22 ± 9.21, and 287.58 ± 10.56 mM, respectively. While vancomycin, clindamycin and amoxicillin showed competitive inhibition the other drugs displayed noncompetitive inhibition.
Related content
Access to resources
Recommend
By Ciftci Mehmet Yilmaz Hayrullah Coban T. Abdulkadir Gul Mustafa Gumustekin Kenan Dane Senol
Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 23, Iss. 2, 2008-04 ,pp. :
By Senturk Murat Irfan Kufrevioglu O. Ciftci Mehmet
Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 24, Iss. 2, 2009-04 ,pp. :