Author： Akkemik Ebru Budak Harun Ciftci Mehmet
Publisher： Informa Healthcare
ISSN： 1475-6366
Source： Journal of Enzyme Inhibition and Medicinal Chemistry, Vol.25, Iss.4, 2010-08, pp. : 476-479
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
The inhibitory effects of some drugs on 6-phosphogluconate dehydrogenase from human erythrocytes have been investigated. For this purpose, initially, erythrocyte 6-phosphogluconate dehydrogenase was purified 3364 times in a yield of 58% by using ammonium sulfate precipitation and 2′,5′-ADP Sepharose 4B affinity gel. A temperature of +4°C was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm. This method was utilized for all kinetic studies. Many commonly used drugs were investigated in this study. Some drugs (ketotifen (Ki: 8.3 ± 1.7 μM), dacarbazine (Ki: 10.1 ± 0.7 μM), meloxicam (Ki: 50.9 ± 13.2 μM), furosemide (Ki: 127 ± 37.8 μM), methotrexate (Ki: 136.7 ± 25.3 μM), metochloropramide hydrochloride (Ki: 2.1113 ± 0.6979 mM), ritodrine hydrochloride (Ki: 6.0353 ± 1.2783 mM), and
Related content
Access to resources
Recommend
By Ciftci Mehmet Yilmaz Hayrullah Coban T. Abdulkadir Gul Mustafa Gumustekin Kenan Dane Senol
Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 23, Iss. 2, 2008-04 ,pp. :
By Senturk Murat Irfan Kufrevioglu O. Ciftci Mehmet
Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 24, Iss. 2, 2009-04 ,pp. :