Mathematical modeling of lipase and protease production by Penicillium restrictum in a batch fermenter

Author： Freire Denise Sant'Anna Geraldo Alves Tito

ISSN： 0273-2289

Source： Applied Biochemistry and Biotechnology, Vol.79, Iss.1-3, 1999-03, pp. : 845-855

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

This work presents a mathematical model that describes time course variations of extracellular lipase and protease activities for the batch fermentation of the fungus Penicillium restrictum, a new and promising strain isolated from soil and wastes of a Brazilian babassu coconutoil industry. The fermentation process was modeled by an unstructured model, which considered the following dependent variables: cells, fat acid, dissolved oxygen concentrations, lipase and protease activities, and cell lysate concentration. The last variable represents the amount of cells that has been lysed by the shear stress and natural cell death. Proteases released to the medium, as consequence of this process, enhance lipase inactivation. The model is able to predict the effects of some operation variables such as air flow rate and agitation speed. The mathematical model was validated against batch-fermentation data obtained under several operating conditions. Because substrate concentration has antagonistic effects on lipase activity, a typical optimization scheme should be developed in order to minimize these deleterious effects while maximizing lipase activity.

Related content

Lipase production byPenicillium restrictum in a bench-scale fermenter: Effect of carbon and nitrogen nutrition, agitation, and aeration

By Freire Denise Teles Elaine Bon Elba Anna Geraldo

Applied Biochemistry and Biotechnology, Vol. 63, Iss. 1, 1997-03 ,pp. : 409-421 (13)

Humana Press, Inc

Access to resources Recommend Favorite

Lipase production by Penicillium restrictum using solid waste of industrial babassu oil production as substrate

By Palma Marcia Pinto Annette Gombert Andreas Seitz Karina Kivatinitz Silvia Castilho Leda Freire Denise

Applied Biochemistry and Biotechnology, Vol. 84, Iss. 1-9, 2000-03 ,pp. : 1137-1145 (9)

Humana Press, Inc

Access to resources Recommend Favorite

Effect of temperature, moisture, and carbon supplementation on lipase production by solid-state fermentation of soy cake by Penicillium simplicissimum

By Luccio Marco Capra Fernando Ribeiro Najara Vargas Gean Freire Denise Oliveira Débora

Applied Biochemistry and Biotechnology, Vol. 113, Iss. 1-3, 2004-03 ,pp. : 173-180 (8)

Humana Press, Inc

Access to resources Recommend Favorite

Purification and Application of a Lipase from Penicillium expansum PED-03

Applied Biochemistry and Biotechnology, Vol. 142, Iss. 2, 2007-08 ,pp. : 194-199 (6)

Humana Press, Inc

Access to resources Recommend Favorite

Production of Penicillium funiculosum 433 Glucose Oxidase and Its Properties

By Sukhacheva M. V. Davydova M. E. Netrusov A. I.

Applied Biochemistry and Microbiology, Vol. 40, Iss. 1, 2004-01 ,pp. : 25-29 (5)

MAIK Nauka/Interperiodica

Access to resources Recommend Favorite