Author： Jatinder K. Chadha B. Saini H.
Publisher： Springer Publishing Company
ISSN： 0959-3993
Source： World Journal of Microbiology and Biotechnology, Vol.22, Iss.1, 2006-01, pp. : 15-22
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Abstract
The medium components for the production of extracellular cellulases by Melanocarpus sp. MTCC 3922 were optimized using solid-state fermentation. Melanocarpus sp. cultured in optimized medium containing 1.5% urea, and 0.12% KH2PO4 along with a trace element solution and surfactant (Tween 20), produced endoglucanase (142.4 U/g of substrate), Avicel-adsorbable endoglucanase (27.0 U/g of substrate), Avicelase (0.65 U/ g of substrate), FPase (39.9 U/g of substrate) and -glucosidase (109.0 U/g of substrate) activities. The presence of sulphate ions in traces stimulated endoglucanase yields. The IEF fractionation of the crude proteins from Melanocarpus sp. showed the expression of 3, 1 and 11 isoforms of endoglucanase, -glucosidase and xylanase, respectively.
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