A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C 13 -norisoprenoid accumulation

Author： Mathieu Sandrine  

Publisher： Oxford University Press

ISSN： 1460-2431

Source： Journal of Experimental Botany, Vol.56, Iss.420, 2005-10, pp. : 2721-2731

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Abstract

A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy--ionone, a C13-norisoprenoidic compound, and a C14-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C13-norisoprenoid level increased from véraison to maturity in both cultivars.

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