Mechanism of High Pressure Denaturation of Proteins

Author: Hayakawa I.   Linko Y.Y.   Linko P.  

Publisher: Academic Press

ISSN: 0023-6438

Source: Lebensmittel-Wissenschaft und -Technologie, Vol.29, Iss.8, 1996-08, pp. : 756-762

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

High pressure denaturation of ovalbumin, bovine serum albumin (BSA) and beta-lactoglobulin was assessed by spectrofluorometry, specific rotation analysis and differential scanning calorimetry (DSC), and compared with heat and chemical denaturation. In all cases the denaturation caused by high pressure was similar to that caused by the cleavage of hydrogen bonds with urea or guanidine hydrochloride.